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Inspection of a random sample of these found two exam ples of why this occurred. BLAST of carp EST EC394432 vs. zebrafish best hit the gene smyhc3 and the reciprocal BLAST vs. carp found EST AB2
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Conditions con sist of an initial denaturation step of 1 min at 95 C, 32 cycles of 30 s at 95 C denaturation, 30 s annealing at 62. 5 C and 25 s elongation at 72 C and a final extension step at